Detection and circulation of hepatitis B virus immune escape mutants among asymptomatic community dwellers in Ibadan, southwestern Nigeria

Simple item page
dc.contributor.authorFaleye TO
dc.contributor.authorAdewumi OM
dc.contributor.authorIfeorah IM
dc.contributor.authorAkere A
dc.contributor.authorBakarey AS
dc.contributor.authorOmoruyi EC
dc.contributor.authorOketunde K
dc.contributor.authorAwonusi OB
dc.contributor.authorAjayi MR
dc.contributor.authorAdeniji JA
dc.date.accessioned2022-07-23T09:28:14Z
dc.date.available2022-07-23T09:28:14Z
dc.date.issued2015
dc.descriptionInternational Journal of Infectious Diseases
dc.description.abstractBackground In 2012, the first Nigerian Hepatitis B Virus (HBV) immune escape mutant (IEM) case was detected in a pregnant woman in southwestern Nigeria. Consequently, this study was designed to investigate the presence and possible circulation of IEMs amongst asymptomatic community dwellers in southwestern Nigeria. Methods Blood specimens collected from 438 asymptomatic community dwellers were screened for HBsAg using ELISA technique. Subsequently, the S-gene was amplified in HBsAg positive samples by a nested PCR protocol, and amplicons sequenced. Isolates were then subtyped by amino acid residues at positions 122, 127, 134 and 160, and genotyped by phylogenetic analysis. Results Of the 31 (7.08%) samples positive for HBsAg, the ∼408bp Sgene fragment was successfully amplified and sequenced in 27. Samples obtained from 4 patients could not be amplified due to low titres. Sequence data from only 15 of the isolates could be analysed further as eight of the remaining 12 had multiple peaks while the rest three showed no similarity to any HBV gene when subjected to BLAST analysis. Thirteen of the 15 isolates were identified as genotype E. Eleven of which were subtyped as ayw4 while the remaining two could not be subtyped due to sR122Q/P substitutions. The last two isolates that could not be genotyped and subtyped had other mutations in the “a” determinant associated with IEMs. Conclusions This study confirmed presence and circulation of HBV IEM in Nigeria, the country's inclusion in the genotype E crescent, and the value of phylogenetic analysis in HBV identification.
dc.identifier.citation10.1016/j.ijid.2015.08.008
dc.identifier.issn1201-9712
dc.identifier.urihttps://nerd.ethesis.ng/handle/123456789/85
dc.language.isoen
dc.subjectHepatitis B virus
dc.subjectHBV
dc.subjectImmune escape mutant
dc.subjectIEM
dc.subjectIbadan
dc.subjectNigeria
dc.titleDetection and circulation of hepatitis B virus immune escape mutants among asymptomatic community dwellers in Ibadan, southwestern Nigeria
dc.typeArticle
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Detection-and-circulation-of-hepatitis-B-virus-immune-_2015_International-Jo.pdf
Size:
1.6 MB
Format:
Adobe Portable Document Format
Download
Collections
Journal Article