Potentials of purified tyrosinase from yam (Dioscorea spp) as a biocatalyst in the synthesis of cross-linked protein networks

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dc.contributor.authorIlesanmi OS
dc.contributor.authorAdedugbe OF
dc.contributor.authorAdewale IO
dc.date.accessioned2022-07-25T22:17:45Z
dc.date.available2022-07-25T22:17:45Z
dc.date.issued2021
dc.descriptionHeliyon
dc.description.abstractWe report the usefulness of yam tyrosinase as a catalyst in the synthesis of cross-linked protein networks for biopolymers. The enzyme was purified using aqueous two-phase partitioning (ATPs) and peptide mapping on SDS-PAGE was carried out to ascertain degree of similarities of tyrosinase from the yam species. The mapping revealed distinct peptide bands of 3, 4, 4 and 2 for tyrosinase from D. praehensilis, D. alata, D. rotundata and C. esculenta respectively purified using conventional method. In contrast, continuous broad band was noticed for the ATPS-purified enzymes due to bound polyethylene glycol (PEG). Tyrosinase from D. praehensilis with overall better properties was used in the synthesis of cross-linked protein networks. The enzyme catalyzed conversion of soluble proteins from whey, moringa leaves, pumpkin leaves and cow blood into fibrous (cross-linked) protein networks for improved properties and functionalities. The purified tyrosinase from D. praehensilis was also covalently bonded to bovine serum albumin (BSA) forming tyrosinase-BSA adduct with molecular weight of 118 ± 2.0 kDa, revealing its potential as a reporter enzyme by reporting BSA. The overall result further reinforces yam tyrosinase as an enzyme of interest in various biotechnological applications.
dc.identifier.citation10.1016/j.heliyon.2021.e07831
dc.identifier.issn2405-8440
dc.identifier.urihttps://nerd.ethesis.ng/handle/123456789/219
dc.language.isoen
dc.subjectTyrosinase
dc.subjectPhase-partitioning
dc.subjectCross-linking
dc.subjectReporter enzyme
dc.subjectYam species
dc.titlePotentials of purified tyrosinase from yam (Dioscorea spp) as a biocatalyst in the synthesis of cross-linked protein networks
dc.typeArticle
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