Detection and molecular characterisation of extended-spectrum β-lactamase-producing enteric bacteria from pigs and chickens in Nsukka, Nigeria

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dc.contributor.authorChah KF
dc.contributor.authorUgwu IC
dc.contributor.authorOkpala A
dc.contributor.authorAdamu KY
dc.contributor.authorAlonso CA
dc.contributor.authorCeballos S
dc.contributor.authorNwanta JN
dc.contributor.authorTorres C
dc.date.accessioned2022-07-23T10:38:05Z
dc.date.available2022-07-23T10:38:05Z
dc.date.issued2018
dc.descriptionJournal of Global Antimicrobial Resistance
dc.description.abstractObjectives This study screened chickens and pigs slaughtered for human consumption for the presence and characteristics of extended-spectrum β-lactamase (ESBL)- and plasmid-encoded AmpC (pAmpC) β-lactamase-producing enteric bacteria. Methods Faecal samples from 410 broiler chickens and 100 pigs were cultured on MacConkey agar supplemented with 2μg/mL cefotaxime. Antimicrobial resistance phenotypes of the recovered isolates were determined by disk diffusion. PCR and sequencing were performed to identify the ESBL and pAmpC gene variants and other associated resistance determinants. Genetic diversity of the isolates was analysed by phylotyping and multilocus sequence typing. Results ESBL-producing Escherichia coli, Klebsiella pneumoniae, Enterobacter asburiae and Providencia spp. were isolated from 17 (4.1%) and 2 (2.0%) of the samples from chickens and pigs, respectively. One pAmpC-producing E. coli isolate was obtained from a chicken. Resistance to tetracycline, trimethoprim/sulfamethoxazole, chloramphenicol and gentamicin was exhibited by 95%, 80%, 60% and 55% of the ESBL/pAmpC-producing strains, respectively. tet(A) and aac(3)-II were the predominant genes detected in tetracycline- and aminoglycoside-resistant strains, respectively. blaCTX-M, encoding CTX-M-15 (15 isolates) or CTX-M-1 variants (3 isolates), was present in all but one ESBL-producer, either alone or in combination with blaSHV and/or blaTEM. The remaining ESBL-producer, a Providencia spp. recovered from a chicken, harboured blaVEB. The only pAmpC-positive E. coli strain carried blaCMY-2. The 11 ESBL-producing E. coli strains belonged to five lineages (ST226-A, ST3625-B1, ST10-A, ST46-A and ST58-B1). Conclusions Healthy chickens and pigs act as reservoirs of ESBL/pAmpC-producing enterobacteria that can potentially be transmitted to humans through direct contact or ingestion of contaminated meat.
dc.identifier.citation10.1016/j.jgar.2018.06.002
dc.identifier.issn2213-7165
dc.identifier.urihttps://nerd.ethesis.ng/handle/123456789/139
dc.language.isoen
dc.subjectEnterobacteriaceae
dc.subjectESBL
dc.subjectpAmpC
dc.subjectFarm animals
dc.titleDetection and molecular characterisation of extended-spectrum β-lactamase-producing enteric bacteria from pigs and chickens in Nsukka, Nigeria
dc.typeArticle
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