Browsing by Author "Owoyele BV"

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    Honey reverses disease progression, has anti-nociceptive and anti-inflammatory effects in a rat model of knee osteoarthritis induced by monosodium iodoacetate
    (2021) Jimoh-Abdulghaffaar HO; Owoyele BV
    Summary Background Clinically, osteoarthritis manifests as joint pain with concomitant loss of joint function, which may ultimately result in a substantially reduced quality of life for the patient. Although, a lot is known about the symptom of the disease, the pathophysiology behind the structural changes is complex and poorly understood. By understanding the mechanisms driving joint tissue destruction in osteoarthritis and identifying the key factors involved, new targets for therapy are emerging that will go beyond symptomatic relief to slowing or stopping the progression of osteoarthritis. Aim Thus, the aim of this study was to evaluate the effect of honey on disease progression, pain perception and inflammation in monosodium iodoacetate (MIA)-induced knee osteoarthritis in female Wistar rats. Methods Thirty, twelve-month old female Wistar rats, weighing between 200 g & 250 g, were randomly divided into five groups of six animals each. Animals in group one were not induced and served as the control, while animals in groups two to five were injected with monosodium iodoacetate in the right knee. In addition, animals in group two received normal saline (1 ml/kg b.w.), group three received arthocare (glucosamine/chondroitin sulphate 6.67/8.33 mg/kg b.w.), group four received low dose honey (250 mg/kg b.w.) while group five received high dose honey (1,000 mg/kg b.w.) and were treated for twenty one days. All animals were subjected to assessment of tactile allodynia (von Frey test), acute inflammation (knee edema), and serum biomarkers: tumour necrosis factor-alpha (TNF-α), vascular endothelial growth factor (VEGF), prostaglandin E2 (PG E2) & cartilage oligomeric matrix protein (COMP) as well as histo-pathological assessment of the right knee joint. Results Honey (at high and low doses), significantly (p < 0.05) reduced tactile allodynia on von Frey test (60 ± 20g) in animals subjected to experimental knee osteoarthritis induced by MIA. Knee edema was also significantly (p < .05) reduced by both high and low doses of honey. Low dose honey significantly (p < .05) reduced he serum levels of TNF-α (61.5 ± 22 pg/ml), VEGF (31 ± 6.1 pg/ml) and COMP (41 ± 14 ng/ml) but, had no effect on the serum level of PG E2. High dose honey on the other hand, only significantly reduced the serum level of TNF-α (87 ± 22 pg/ml) but, had no effect on the serum levels of VEGF, COMP and PG E2. However, the administration of honey did not show any significant effect on histo-pathological features of the induced osteoarthritis. Conclusion Honey reversed disease progression and reduced pain perception as well as inflammation in MIA-induced knee osteoarthritis in female Wistar rats. However, honey had no effect on the histo-pathology of the knee joint.
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    Therapeutic effects of crude extracts of Bacopa floribunda on beta-amyloid 1-42-induced Alzheimer’s disease via suppression of dyslipidemia, systemic inflammation and oxidative stress in male Wistar Rats
    (2022) Oyeleke MB; Oni HT; Arokoyo OL; Owoyele BV
    Aims Bacopa floribunda (BF), an African traditional plant and its species have been widely used as brain tonic for memory enhancement. It has also been reported to help relieve anxiety and some psychological disorders. This study aimed to investigate the mechanisms of action of BF on Amyloid beta (Aβ) 1–42 peptides induced cognitive deficit in male Wistar rats. Main methods A total of 48 healthy male wistar rats were used for this study. Some groups were pre-treated with 200 mg/kg of BF extracts before a single bilateral injection of Aβ 1–42 while some were post-treated with BF for 21 days after Aβ1-42 exposure. Cognitive performance was evaluated using Y-Maze and Novel Object recognition tests. After treatments, hippocampal homogenates were assayed for the levels of Acetylcholinesterase, Na–K/ATPase activities, glutamate and Aβ1-42 concentrations among others. Key findings It was observed that Aβ1-42 caused cognitive impairment and BF extracts especially the ethanol extract was able to significantly (p < 0.05) reverse almost all the perturbations including lipid imbalance caused by Aβ1-42 assault mainly at the post-treatment level. Significance Administration of ethanol and aqueous extracts of BF mitigated the hazardous effect of Aβ1-42 observed in the blood plasma and hippocampal homogenates. In this context, we conclude that BF is an efficient cognitive enhancer that can help alleviate some symptoms associated with Alzheimer’s disease.