Ladoke Akintola University of Technology

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Browsing Ladoke Akintola University of Technology by Author "Adebayo EA"

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    Bio-physical effects of radiofrequency electromagnetic radiation (RF-EMR) on blood parameters, spermatozoa, liver, kidney and heart of albino rats
    (2019) Adebayo EA; Adeeyo AO; Ogundiran MA; Olabisi O
    Current study aimed at determining the consequence of Radiofrequency (≈1800 MHz) electromagnetic radiation (RF-EMR) on the histological, hematological and histochemical properties of selected tissues of rat and to assess morphological changes associated with such exposures. Three groups of thirty rats of which two groups of twenty rats were subjected to average radiation of ≈1.40 W/cm2 at 24 m from the base of two different telecommunications network mast for 5 weeks. Histochemical, hematological and histological analyses of the rats were afterward carried out on selected tissues of the experimental animals using standard procedures. Impacts of radiation were noted in the exposed groups of rats. There was rise in white blood cells with variations in other blood parameters; the sperm motility and sperm count of the exposed rats is lower than the control; the liver of the exposed rats shows pronounced dilated sinusoids, distorted architecture, hyperchromatic nuclei, congested central vein, with change of hepatocytes structure; the heart shows gross distortion of cardiac muscular architecture with distorted irregular cardiac muscle fibres and wider interfibres spaces; kidney showing hyperchromatic nuclei, gradual loss and degeneration of flattened squamous epithelial cells lining and testis shows grossly distorted seminiferous tubules and epididymis with loss of cellular structure and an area of inflammatory changes with complete absence of spermatozoa, which may lead to low fertility. This study shows that radiofrequency electromagnetic radiation may induce morphological changes in various tissues of living systems in rat.
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    Photo-assisted bio-fabrication of silver nanoparticles using Annona muricata leaf extract: exploring the antioxidant, anti-diabetic, antimicrobial, and cytotoxic activities
    (2020) Badmus JA; Oyemomi SA; Adedosu OT; Yekeen TA; Azeez MA; Adebayo EA; Lateef A; Badeggi UM; Botha S; Hussein AA; Marnewick JL
    Green synthesis of metal nanoparticles is reputed to have a robust range of biomedical applications. Silver nanoparticles (AgNPs) bio-fabricated using aqueous leaf extract of Annona muricata were characterized and evaluated for in-vitro antioxidant, lipid peroxidation inhibition, anti-diabetic and antimicrobial activities as well as cytotoxicity in human keratinocyte cells (HaCaT). The extract induced colour change of silver salt solution which absorbed at 420 nm and confirmed the formation of AgNPs. FTIR showed that free amide and hydroxyl groups were responsible for the synthesized nanoparticles. Both XRD and SAED confirmed the crystalline nature of the particles with face centered cubic (FCC) phase. The zeta potential revealed -27.2 mV potential and average distribution size of 35 nm. DLS indicated that the majority of the particles were 86.78 nm size and with a polydispersity index (PDI) of 0.329. AgNPs displayed strong activities against DPPH (IC50 = 51.80 μg/ml), ABTS (IC50 = 30.78 μg/ml), α-amylase (IC50 = 0.90 μg/ml) and α-glucosidase (IC50 = 3.32 μg/ml). The particles exhibited a dose-dependent inhibition of Fe2+-induced lipid peroxidation with effective antimicrobial activity against a battery of bacterial strains and cytotoxicity in HaCaT cell line. These findings revealed the potential biomedical applications of the particles and further work will be required to establish its molecular mechanism of action.