Ladoke Akintola University of Technology

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Browsing Ladoke Akintola University of Technology by Author "Abijo AZ"

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    Biochemical and histological investigation on the protective effect of poly-herbal extract in high salt diet-fed male Wistar rats
    (2021) Olorunnisola OS; Adegbola PI; Ajilore BS; Abijo AZ; Ajayi FA; Fadahunsi OS
    Background Through different mechanisms, excessive intake of dietary salt has been documented as a risk factor for the development of elevated blood pressure and organ dysfunction. Therefore, this study evaluated the efficacy of combined extracts of four medicinal plants against high salt diet-induced succinate dehydrogenase depletion and alteration in vital organ (kidney, liver, and cardiac) hemostasis. Methods Thirty-five male Wistar rats with an average weight of 130 g were divided into 5 groups of 7 animals each. Group 1 and 2 rats were fed with normal rat chow (NRC) and 16% high salt diet (HSD) only respectively. Rats in groups 3, 4, and 5 were fed with 16% HSD and 800, 400, and 200 mg/kg poly-herbal extract (PHE), respectively, once daily for 4 weeks. The concentration and activities of creatinine, urea, sodium, magnesium, chloride, potassium, albumin, globulin, total protein, and alkaline phosphate (ALP) were estimated in the serum. Aspartate and alanine aminotransferases (AST and ALT), lactate dehydrogenase (LDH), succinate dehydrogenase (SDH) were estimated in the serum, liver, and aorta. The result revealed that the combined extract significantly (p < 0.05) lowered HSD-induced elevated serum concentration of kidney function markers and electrolytes. More so, a significant (p < 0.05) reduction in serum and tissue activities of liver and cardiac function markers was demonstrated by the poly-herbal extract. No significant changes were observed in serum albumin and globulin level, however total protein and tissue activities of SDH were significantly (p < 0.05) elevated by the PHE. The histopathological examination showed to a large extent, the extract conferred significant protection on the cytoarchitecture of the kidney, liver, and aorta. Conclusion Conclusively, combined plants extract protected against high salt diet-induced biochemical and histological derangement in male Wistar rats.
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    Computational prediction of nimbanal as potential antagonist of respiratory syndrome coronavirus
    (2021) Adegbola AE; Fadahunsi OS; Alausa A; Abijo AZ; Balogun TA; Aderibigbe TS; Semire B; Adegbola PI
    The high pathogenic nature of the Middle East Respiratory coronavirus (MER) and the associated high fatality rate demands an urgent attention from researchers. Because there is currently no approved drug for the management of the disease, research efforts have been intensified towards the discovery of a potent drug for the treatment of the disease. Papain Like protease (PLpro) is one of the key proteins involved in the viral replication. We therefore docked forty-six compounds already characterized from Azadirachta indica, Xylopia aethipica and Allium cepa against MERS-CoV-PLpro. The molecular docking analysis was performed with AutoDock 1.5.6 and compounds which exhibit more negative free energy of binding, and low inhibition constant (Ki) with the protein (MERS-CoV-PLpro) were considered potent. The physicochemical and pharmacokinetic properties of the compounds were predicted using the Swissadme web server. Twenty-two of the compounds showed inhibition potential similar to dexamethasone and remdesvir, which had binding affinity of −6.8 and −6.3 kcal/mol respectively. The binding affinity of the compounds ranged between −3.4 kcal/mol and −7.7 kcal/mol whereas; hydroxychloroquine had a binding affinity of −4.5 kcal/mol. Among all the compounds, nimbanal and verbenone showed drug likeliness, they did not violate the Lipinski rule neither were they inhibitors of drug-metabolizing enzymes. Both nimbanal and verbenone were further post-scored with MM/GBSA and the binding free energy of nimbanal (−25.51 kcal/mol) was comparable to that of dexamethasone (−25.46 kcal/mol). The RMSD, RMSF, torsional angle, and other analysis following simulation further substantiate the efficacy of nimbanal as an effective drug candidate. In conclusion, our study showed that nimbanal is a more promising therapeutic agent and could be a lead for the discovery of a new drug that may be useful in the management of severe respiratory coronavirus syndrome.