Isolation and characterisation of colistin-resistant Enterobacterales from chickens in Southeast Nigeria

Simple item page
dc.contributor.authorAnyanwu MU
dc.contributor.authorMarrollo R
dc.contributor.authorPaolucci M
dc.contributor.authorBrovarone F
dc.contributor.authorNardini P
dc.contributor.authorChah KF
dc.contributor.authorShoyinka SV
dc.contributor.authorCarretto E
dc.date.accessioned2022-07-23T10:38:06Z
dc.date.available2022-07-23T10:38:06Z
dc.date.issued2021
dc.descriptionJournal of Global Antimicrobial Resistance
dc.description.abstractABSTRACT Objectives Resistance to colistin (CST) mediated by mobile genetic elements has had a broad impact worldwide. There is an intensified call for epidemiological surveillance of mcr in different reservoirs to preserve CST for future generations. In Nigeria, the poultry industry is a key livestock sector. This study was undertaken to screen putative colistin-resistant Enterobacterales (CST-r-E) from poultry birds in Southeast Nigeria and to determine the genetic relatedness of mcr-harbouring isolates. Methods Faecal and cloacal swab samples (n = 785) were collected from chickens in 17 farms located in three contiguous states in Southeast Nigeria between March–November 2018. Following selective culture, CST-r-E were isolated. Confirmation of CST resistance, antimicrobial susceptibility testing, molecular detection of genes mcr-1 to mcr-10, multilocus sequence typing (MLST) and randomly amplified polymorphic DNA (RAPD) analysis were performed on the isolates. A questionnaire was distributed to investigate the knowledge about CST and its use of chicken farm caretakers. Results Of the 785 samples evaluated, 45 (5.7%) were positive for 48 CST-r-E, among which 23 harboured the mcr-1 gene (22 Escherichia coli and 1 Klebsiella pneumoniae). In two E.coli isolates, a new allelic variant (mcr-1.22) was detected. RAPD analysis allowed the identification of 11 different fingerprints. MLST also revealed 11 STs, with 3 of them being novel. Conclusion mcr has significantly spread in poultry birds of Southeast Nigeria, which poses a worrisome risk to veterinary and human health. Strategies to prevent indiscriminate use of CST in farms should be quickly adopted before CST resistance becomes a huge global health issue.
dc.identifier.citation10.1016/j.jgar.2021.04.030
dc.identifier.issn2213-7165
dc.identifier.urihttps://nerd.ethesis.ng/handle/123456789/151
dc.language.isoen
dc.subjectAntimicrobial resistance
dc.subjectEnterobacterales
dc.subjectNigeria
dc.subjectmcr-1
dc.subjectMobile colistin resistance
dc.subjectmcr-1.22
dc.titleIsolation and characterisation of colistin-resistant Enterobacterales from chickens in Southeast Nigeria
dc.typeArticle
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Isolation-and-characterisation-of-colistin-resista_2021_Journal-of-Global-An.pdf
Size:
1 MB
Format:
Adobe Portable Document Format
Download
Collections
Journal Article